For Runabout to be useful, a number of experiments must be performed on a uniformly-13C/15N- labeled polypeptide. At minimum, these include the HNCO, HNCACB, and CBCACONH (aka HNCOCACB). Redundant spectral information, however, make assignment much easier, so several more experiments should, if possible, be performed. These include, in order, the HNCA, HNCACO, HNCOCA. The CCONH should also be considered, as it can readily identify the amino acid type of many residues by their 13C chemical shift patterns.

Extra care should be taken to acquire high-quality data for the HNCO, as it will be used as the reference spectrum. Also, little progress will be made if the signals are weak in the HNCACB and HNCOCACB/CBCACONH experiments. When budgeting experiment time, it will not make sense to skimp on these experiments.